Principles and techniques of digital holographic microscopy. Instead, the light wave front information originating from the object is digitally r. Low coherent microscopy and optical trapping in nanooptics and biomedical metrology springer series in optical sciences 221 stuerwald, stephan on. Digital holography aims at recording, on a lensless imaging sensor, the interferences between the light field diffracted, reflected or scattered by an object and a reference beam. Digital holographic microscopy dhm is an emerging highresolution imaging technique that offers realtime imaging and quantitative measurements of physiological parameters without any staining or. Digital holographic microscopes can be built in either reflection or transmission modes and can be used for detection in either the image digital holographic microscopy dhm or fourier digital fourier holographic microscopy dfhm planes. In digital holographic microscopy, a single hologram is used to numerically focus on the holographic image at any distance 18,19.
Digital holographic microscopy dhm presented in this paper takes advantage of both digital holography and microscopy with a micro objective mo in the object beam of the digital hologram recording system. Nowadays, conventional research microscopes include a digital camera to take digital images of what is being analyzed. The usefulness of digital holographic microscopy for. The digital holographic microscopy dhm as a patented technology makes use of a video ccd camera to record a hologram produced by the interference between a reference wave and a wave emanating from the specimen. Dhm is quite similar to conventional microscopy, which you might have used in your biology classes at highschool.
Both the amplitude and phase of the electric field originating from the sample are reconstructed at the imaging sensor, affording a gateway to advanced imaging methodologies, such as phase imaging, 3d imaging, and metrology. Digital holographic microscopy dhm is a labelfree imaging technique allowing visualization of transparent cells with classical imaging cell culture plates. Robust phase aberration compensation in digital holographic. Digital holographic microscope dhm can digitally record and reconstruct the wavefronts information of the specimens, the amplitude and phase information of the waves can be quantitatively retrieved accurately and in near realtime, so that it has been a powerful tool for labelfree quantitative phase imaging qpi. Digital holographic microscopes vincent m rossi digital holography has evolved as a robust and informative means of microscopy.
Enter your mobile number or email address below and well send you a link to download the free kindle app. Grosfils, darkfield digital holographic microscopy to investigate objects that are nanosized or smaller than the optical resolution, optics letters 33, 26052607 2008. Review of quantitative phasedigital holographic microscopy. A state of the art presentation of important advances in the field of digital holography, detailing advances related to fundamentals of digital holography, inline holography applied to fluid mechanics, digital color holography, digital holographic microscopy, infrared holography, special techniques in full field vibrometry and inverse problems in digital holography. Pdf lensless multispectral digital inline holographic microscope. Digital holography has evolved as a robust and informative means of microscopy. The last chapter discusses speckle metrology techniques and compares them to digital holographic. The quantitative dhm phase contrast image provided is related both to the intracellular refractive index and to cell thickness. Digital holographic interference microscopy dhim is a combination of the holographic interference microscopy with digital methods of image processing for 3d imaging of phase microobjects. Tracking with superresolution digital holographic microscopy.
This spotlight will begin with an introduction to dhm via a brief history of holography. Digital holographic microscopy is a fast threedimensional 3d imaging tool with many applications in soft matter physics. By replacing the photochemical procedures with electronic imaging and having a direct numerical access to the complex optical field, a wide range of new imaging capabilities become available, many of them difficult or infeasible in conventional holography. A method of choice among available techniques is digital holography, which consists of a 3d timeresolved quantitative imaging system.
In this chapter, we show the capability of reconstructing digital holograms at different. This spotlight begins with an introduction to digital holographic microscopy via a brief history of holography. Schematic of the digital holographic microscopy setup used in this work. Digital holographic microscopy dhm is a very popular noninvasive testing tool. Direct access to the phase information leads to quantitative phase microscopy with nanometer. The aim of this paper is to introduce digital holographic microscopy dhm as a noncontact, inexpensive, and nonabrasive method for 3d surface characterization of polymeric nanocomposites. Digital holographic interferometry is discussed in chapter 4. Then you can start reading kindle books on your smartphone, tablet, or computer no kindle device required. Digital holographic microscopy dhm is a type of microscopy that combines digital holography with microscopy to allow transparent cells to be. Digital holographic microscopy dhm is digital holography applied to microscopy.
Principles, techniques, and applications springer series in optical sciences by myung k. The last chapter discusses speckle metrology techniques and. New techniques in digital holography wiley online books. Digital holographic dh microscopy is a digital highresolution holographic imaging technique with the capacity of quantification of cellular conditions without any staining or labeling of cells. Feb 26, 2019 digital holographic microscopy dhm is a type of microscopy that combines digital holography with microscopy to allow transparent cells to be visualized with classic cell culture plates. Digital holographic microscopy wikimili, the free encyclopedia. Measuring dynamics and interactions of colloidal particles with digital holographic. Pdf an compact multispectral digital inline holographic microscope dihm is developed that emulates gabors original holographic principle. While phasesensitive imaging methodologies such as darkfield, phase contrast and differential interference contrast are known for several decades, they cannot provide. It first provides an overview of digital holographic microscopy dhm and holographic optical tweezers as well as nondiffracting beam types for minimalinvasive, realtime and markerfree imaging as well as manipulation of micro and nano objects. Special applications and techniques like short coherence recording, particle field applications, and comparative holography are discussed in chapter 6. Digital holographic microscopy dhm is an emerging highresolution imaging technique that offers realtime imaging and quantitative measurements of physiological parameters without any staining or labeling of cells.
Digital holographic microscopy principles, techniques. Marquet p1, rappaz b, magistretti pj, cuche e, emery y, colomb t, depeursinge c. Digital holographic microscopy dhm lets focus on the dhm technique that i mentioned before. The first dhm images of living cells were obtained 810 years ago 1, 2. Principles, techniques, and applications springer series in optical sciences book 162 kindle edition by kim, myung k. The captured image is transmitted to a computer where numerical procedures are applied to reconstruct a 3d image of the specimen. Lensless imaging refers to an imaging technique which requires no imaging element between the.
Use features like bookmarks, note taking and highlighting while reading digital holographic microscopy. Autofocusing in digital holographic microscopy article pdf available in 3d research 21. First, the basic results from the general theory of holography, based on the scalar diffraction theory, are summarized, and a general description of the digital holographic microscopy process is given, including quantitative phase microscopy. The fifth chapter is dedicated to digital holographic microscopy. The second chapter discusses the different options and modalities available when designing a digital holographic microscope dhm, including advantages. Digital holographic microscopy with extended field of view using.
Recent studies have shown that electromagnetic scattering solutions can be fit to digital holograms to obtain the 3d positions of isolated colloidal spheres with nanometer precision and millisecond temporal resolution. Digital holographic microscopy is an emerging modality that offers capability of quantitative phase imaging qpi of transparent unstained cells in their most natural state. Principles, techniques, and applications springer series in optical. There is also a chapter devoted to digital holographic interferometry with applications in holographic microscopy, quantitative phase contrast imaging, multidimensional deformation investigations. Low cost digital holographic microscope for 3d cell imaging by. Digital inline holographic microscopy jorge garciasucerquia, wenbo xu, stephan k.
Digital holography and its multidimensional imaging. Instead, the light wave front information originating from the object is digitally recorded as a hologram, from which a computer calculates the object image by using a numerical reconstruction algorithm. The unique measurable parameters are the cell number, cell area, thickness, and volume, which can be coupled to proliferation, migration, cell cycle analysis, viability, and cell death. Kim, is intended to provide a brief but consistent introduction to the principles of digital holography as well as to give an organized overview of the large number of techniques and applications being developed. Microtopography and thickness measurement with digital. Signal processing challenges for digital holographic video. Design, characterization, and image reconstruction. Cells and holograms holograms and digital holographic. Spatiotemporal multiplexing in digital holographic microscopy and holographic displays digital holography and threedimensional imaging, optical society of america 2017, p. Digital holographic microscopy dhm is a wellestablished technique for quantitative phase measurements 1, 2, 3. Digital holographic microscopy dhm is an emerging highresolution imaging technique. Digital holography is an optical tool that is used to reconstruct the phase and intensity maps of the tested objects simultaneously. Digital holographic microscopy distinguishes itself from other microscopy methods by not recording the projected image of the object.
She is an author of one monograph, several book chapters, and. There is also a chapter devoted to digital holographic interferometry with applications in holographic microscopy, quantitative phase contrast imaging, multidimensional deformation investigations, surface shape measurements, fluid mechanics, refractive index investigations, synthetic aperture imaging and information encrypting. Tip crack imaging on transparent materials by digital. The hologram results from the interference of the object beam. Digital holographic microscopy for3d imaging of complex. The holographic phasecontrast or interferencecontrast images interferograms are recorded by a digital camera from which a computer reconstructs 3d. Digital holography is an emerging field of new paradigm in general imaging applications. Apr 01, 2010 digital holography is an emerging field of new paradigm in general imaging applications. Digital holographic dh microscopy is a digital highresolution. Principles, techniques, and applications springer series in optical sciences book 162 kindle edition by kim, myung k download it once and read it on your kindle device, pc, phones or tablets.